115 Effects of Probiotic and Heat-Killed Probiotic Supplementation on Systemic Biomarkers of Oxidative Stress and Inflammation and Fermentative end Products of Adult Cats

Abstract Probiotics and heat-killed probiotics (postbiotic) have been linked with enhancement of the intestinal barrier function mucosal immunity, anti-inflammatory properties, as well production fermentative end-products including short-chain fatty acids (SCFA), which may confer benefits to gut microbiome health. This study aimed evaluate longitudinal effects daily supplementation pro- post-biotics on oxidative stress biomarkers such malondialdehyde (MDA) superoxide dismutase (SOD), inflammatory from a panel 19 cytokines chemokines, hormone leptin, fecal metabolites, phenol indole, SCFA, ammonia, adult cats. The was complete randomized design 36 cats divided into 3 treatments (n=12/treatment). Animals were fed standard extruded commercial diet. used control diet + placebo (maltodextrin carrier; CON), Probiotic (Bifidobacterium animalis subsp. Lactis CECT8145; dosage: 109CFU/cat; PRO), postbiotic (Heat-killed Bifidobacterium lactis PST). Test articles supplied by ADM Biopolis (Valencia, Spain). Longitudinal analyses performed every 30 days for total 90 days. No interaction day treatment (P >0.05) observed any variables evaluated. For there main effect butyrate concentration, PST greater (P< 0.05) than PRO. It also some CON had reduced GM-CSF, KC, SDF-1, when compared PST, PRO, both PRO respectively. Whilst RANTES IL-12(p40), concentration Overall, probiotic showed no detrimental biomarkers, in